PreCursor-M AnoGYN

PreCursor-M AnoGYN is a methylation-specific molecular assay to detect promoter hypermethylation of the human genes ASCL1 and ZNF582  in bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens.

Special Features:

  • One-step PCR:
    • Multiplex format enabling simultaneous amplification and detection of the two targets and controls in a single reaction
  • Ready-to-use mastermix:
    • Just add bisulfite-converted DNA
  • Reliable:
    • Robust
    • Good reproducibility
    • Built-in check for sample quality and normalization.
Contact sales for information
For research use only
PreCursor-M AnoGYN

Product number 81524

72 Tests
Please contact your local Fujirebio representative for the availability of this product in your country.

Click here to navigate

  • Contact sales for information
  • Details
  • Conditions of sale
  • Documentation
  • Insights
  • Product inquiry
  • Related products

  • Details

    • Method: Multiplex real-time methylation-specific PCR assay
    • Target: Human genes ASCL1 and ZNF582
    • Built-in reference and sample quality control: β-actin (ACTB)
    • Calibrator: Contains a standard concentration of low-copy DNA fragments of the two targets ASCL1, ZNF582 and the control gene ACTB – also used for run validation
    • Sample types: Bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens
    • Sample volume: 5 μL of bisulfite-converted DNA
    • Total time to result: Ranging between 2 to 24 hours depending on the level of automation (incl. DNA extraction and bisulfite conversion)
    • Equipment: Mic qPCR cycler (Bio Molecular Systems), Rotor-Gene Q 5plex system (Qiagen) or ViiA 7 qPCR system (Thermo Fisher Scientific)

     

    References:

    1. Costello, J.F., and Plass C. (2001) Methylation matters. J. Med. Genet. 38, 285-303.
    2. van der Zee R.P. , et al. Host cell deoxyribonucleic acid methylation markers for the detection of high-grade anal intraepithelial neoplasia and anal cancer. Clin. Infect. Dis., 68 (7); 1110-1117, 2019
    3. van der Zee R.P. , et al. Cancer risk stratification of anal intraepithelial neoplasia in human immunodeficiency virus-positive men by validated methylation markers associated with progression to cancer. Clin. Infect. Dis., 72 (12); 2154-2163, 2021
    4. van der Zee R.P. , et al. DNA methylation markers have universal prognostic value for anal cancer risk in HIV-negative and HIV-positive individuals. Mol. Oncol., 15 (11); 3024-3036, 2021, 2021
    5. Rozemeijer K, et al. Analytical validation and diagnostic performance of the ASCL1/ZNF582 methylation test for detection of high-grade anal intraepithelial neoplasia and anal cancer. Tumour Virus Res. 30;17:200275, 2023
    6. Steenbergen, R.D.M. et al. (2014) Clinical implications of (epi)genetic changes in HPV-induced precancerous lesions. Nat. Rev. Cancer 14, 395–405.

  • Conditions of sale

    To read the end user conditions of sale for this product please visit our Resource center.

  • Insights

  • Product inquiry

    CAPTCHA

    The provision of your data implies your consent to the processing of your data in our database and, depending on the option you choose, to receiving our monthly newsletter by e-mail.

    For more information on how we process your personal data and regarding the rights you have in this respect, we refer to our privacy policy, which we hold at your disposal HERE.

    If you no longer wish to receive our newsletter, you have the option to unsubscribe via the ‘unsubscribe’ tool at the bottom of the newsletter. If you want to have your data removed from our database, please contact us at: privacy@fujirebio.com.

Veuillez confirmer que vous êtes un professionnel de la santé

Le contenu de ce site web est en partie réservé aux professionnels de santé qui sont directement impliqués dans la fourniture de services de soins de santé aux patients. Si vous êtes un professionnel de santé et que vous disposez de tous les droits d'accès, veuillez confirmer en cliquant sur le bouton 'oui'.

Choose your country or region

Once you have made this selection we will show you only the content that is relevant for your country. You can change this setting any time by clicking on the flag in the top toolbar of our website and choosing another country.
The content of this website is in part restricted to healthcare professionals who are directly involved in the provision of healthcare services to patients. If you are a health professional and you have full access rights, please check this box and confirm below.